Serratia Marcescens Lab Report Essays

Serratia Marcescens Lab Report Essays Serratia Marcescens Lab Report Paper Serratia Marcescens Lab Report Paper In bacteria, temperature, pH, and other chemical agents a II affect the expression of genes. In this lab, the effect of temperature change on the gene which codes for a red pigment called prognosis of bacterium Seer TIA mercenaries is being tested. Seriate mercenaries is usually found in OSI I and plants, and the accumulation of prognosis in the bacterial cells makes them appear red. Prognosis is produced only at certain temperatures, so b y regulating the temperature in which Seriate mercenaries is cultured, the optimum temperature for the most prognosis to be produced can be tested. Purpose The purpose of this lab is to observe the effect of temperature c anger on the production of the pigment prognosis by the back terbium Seriate mercenaries, and also to determine whether previous culture conditions affect gene expression. Hypothesis If the bacteria is cultured in 27 , then it will produce more prognosis than the bacteria cultured in 37 Regardless of the first culture conditions, the bacteria recaptured in 27 co will produce more prognosis than the bacteria recaptured in 37 Materials see attached lab, materials, page 25 Independent Variable: Temperature (in Celsius) Dependent Variable: Amount of prognosis produced Methods/Procedures see attached lab, procedures, page 2527 Data see attached lab, data, page 25, 26 Analysis 1. You should label the bottom off Petri dish instead of the top because this way, the lids will not accidentally be sit ached. It is also easier to read the label if it is on the bottom, because the Petri dishes will be put into the incubator upside down to minimize condensation. 2. You must not touch a non sterile surface with the applicant or tip before obtaining the innocuous from the stock culture because the applicator tip might become contaminated, which would compromise the experiment. 3. You should lift the lid of the Petri dish only 23 CM rather t Han remove it completely in order to keep as little of any thing other than the bacteria from contaminating or getting into the Petri dish. . If I cultured two samples of bacteria and grew them at 32 co , I predict that the samples will produce less prognosis (be less red) than the bacteria cultured in 27 co , but produce more prognosis (be more red) than the bacteria cultured in 37 co 5. If the new cultured were grown at 37 for 8 hours, then at 27 for 24 hours, I predict hat the bacteria will produce prognosis (be red) because according to the data, the c onditions of the recapture have more of an effect on the production of prognosis than the conditions of the initial culture. . The temperature at which the bacteria were originally re cultured has more effect on the production of prognosis t Han the temperature at which the bacteria were originally cultured. Both samples recaptured in 27 were red, meaning that they produced prognosis, regardless of their initial culture c notations, while both samples recaptured at 37 ere white, signifying the absence of prognosis production, also regardless of their original culture conditions. 7. Prognosis is not only a pigment; it is also an antibiotic. Its function may be to kill other microorganisms which might be harmful to the Seriate mercenaries that live in the same temperature range. 8. An advantage of the temperature sensitivity of prognosis proud action might be that the bacteria would only produce it when needed d. The ability to control the production of prognosis according to temperature helps the bacteria to synthesize the pigment helps it o not produce excess prognosis when it is not needed. Conclusion My hypothesis, which was that the bacteria cultured in 27 co will produce more prognosis than the bacteria cultured in 37 , and also that the bacteria recaptured in 27 (regardless of the original culture conditions) will produce more p Rodings than the bacteria recaptured in 37 (also regardless of the original culture condition s), was supported. The S. Mercenaries cultured in 27 turned red, indicating the production of pro disposing, while the S. Mercenaries cultured in remained white, indicating that no prognosis was synthesized.

Characteristics of Ancient Monumental Architecture

Characteristics of Ancient Monumental Architecture The term monumental architecture refers to large humans include pyramids, large tombs, and burial mounds, plazas, platform mounds, temples and churches, palaces and elite residences, astronomical observatories, and erected groups of standing stones. The defining characteristics of monumental architecture are their relatively large size and their public nature- the fact that the structure or space was built by lots of people for lots of people to look at or share in the use of, whether the labor was coerced or consensual, and whether the interiors of the structures were open to the public or reserved for an elite few.   Who Built the First Monuments? Until the late 20th century, scholars believed that monumental architecture could only be constructed by complex societies with rulers who could conscript or otherwise convince the residents into working on large, non-functional structures. However, modern archaeological technology has given us access to the earliest levels of some of the most ancient tells in northern Mesopotamia and Anatolia, and there, scholars discovered something amazing: monumentally-sized cult buildings were constructed at least 12,000 years ago, by what started out as egalitarian hunters and gatherers. Before the discoveries in the northern Fertile Crescent, monumentality was considered costly signaling, a term that means something like elites using conspicuous consumption to demonstrate their power. Political or religious leaders had public buildings built to indicate that they had the power to do so: they certainly did that. But if hunter-gatherers, who ostensibly didnt have full-time leaders, built monumental structures, why did they that do that? Why Did They Do That? One possible driver for why people first started building special structures is climate change. Early Holocene hunter-gatherers living during the cool, arid period known as the Younger Dryas were susceptible to resource fluctuations. People rely on cooperative networks to get them through times of social or environmental stress. The most basic of these cooperative networks is food sharing. Early evidence for feasting- ritual food sharing- is at Hilazon Tachtit, about 12,000 years ago. As part of a highly organized food-sharing project, a large-scale feast can be a competitive event to advertise community power and prestige. That may have led to the construction of larger structures to accommodate larger numbers of people, and so forth. It is possible that the sharing simply stepped up when the climate deteriorated. Evidence for the use of monumental architecture as evidence for religion usually involves the presence of sacred objects or images on the wall. However, a  recent study by behavioral psychologistsYannick Joye and Siegfried Dewitte (listed in the sources below) has found that tall, large-scale buildings produce measurable feelings of awe in their viewers. When awe-struck, viewers typically experience momentary freezing or stillness. Freezing is one of the main stages of the defense cascade in humans and other animals, giving the awe-struck person a moment of hyper-vigilance toward the perceived threat. The Earliest Monumental Architecture The earliest known monumental architecture is dated to the periods in western Asia known as pre-pottery Neolithic A (abbreviated PPNA, dated between 10,000–8,500 calendar years BCE [cal BCE]) and PPNB ( 8,500–7,000 cal BCE). Hunter-gatherers living in communities such as Nevali Çori, Hallan Çemi, Jerf el-Ahmar, D’jade el-Mughara, Çayà ¶nà ¼ Tepesi, and Tel Abr all built communal structures (or public cult buildings) within their settlements. At Gà ¶bekli Tepe, in contrast, is the earliest monumental architecture located outside of a settlement- where it is hypothesized that several hunter-gatherer communities gathered regularly. Because of the pronounced ritual / symbolic elements at Gà ¶bekli Tepe, scholars such as Brian Hayden have suggested that the site contains evidence of emergent religious leadership. Tracing the Development of Monumental Architecture How cult structures might have evolved into monumental architecture has been documented at Hallan Çemi. Located in southeastern Turkey, Hallan Cemi is one of the oldest settlements in northern Mesopotamia. Cult structures significantly different from regular houses were constructed at Hallan Cemi about 12,000 years ago, and over time became larger and more elaborate in decoration and furniture. All of the cult buildings described below were located at the center of the settlement and arranged around a central open area about 15 m (50 ft) in diameter. That area contained dense animal bone and fire-cracked rock from hearths, plaster features (probably storage silos), and stone bowls and pestles. A row of three horned sheep skulls was also found, and this evidence together, say the excavators, indicates that the plaza itself was used for feasts, and perhaps rituals associated with them. Building Level 3 (the oldest): three C-shaped buildings made of river pebbles about 2 m (6.5 ft) in diameter and mortared with white plasterBuilding Level 2: three circular river-pebble buildings with paved floors, two 2 m in diameter and one 4 m (13 ft). The largest had a small plastered basin in the center.Building Level 1: four structures, all constructed of sandstone slabs rather than river pebbles. Two are relatively small (2.5 m, 8 ft in diameter), the other two are between 5-6 m (16-20 ft). Both of the larger structures are fully circular and semi-subterranean (excavated partly into the ground), each with a distinctive semicircular stone bench set against the wall. One had a complete auroch skull which apparently hung on the north wall facing the entrance. The floors had been resurfaced multiple times with a distinctive thin yellow sand and plaster mixture over a relatively sterile fine dirt fill. Few domestic materials were found inside the structures, but there were exotics, including copper ore and obsidian. Examples Not all monumental architecture was (or is for that matter) built for religious purposes. Some are gathering places: archaeologists consider plazas a form of monumental architecture since they are large open spaces built in the middle of town to be used by everyone. Some are purposeful- water control structures like dams, reservoirs, canal systems, and aqueducts. Sports arenas, government buildings, palaces, and churches: of course, many different large communal projects still exist in modern society, sometimes paid for by taxes. Some examples from across time and space include Stonehenge in the UK, the Egyptian Giza Pyramids, the Byzantine Hagia Sophia, the Qin Emperors Tomb, the American Archaic Poverty Point earthworks, Indias Taj Mahal, Maya water control systems, and the Chavin culture Chankillo observatory. Sources Atakuman, Çigdem. Architectural Discourse and Social Transformation During the Early Neolithic of Southeast Anatolia. Journal of World Prehistory 27.1 (2014): 1-42. Print. Bradley, Richard. Houses of Commons, Houses of Lords: Domestic Dwellings and Monumental Architecture in Prehistoric Europe. Proceedings of the Prehistoric Society 79 (2013): 1-17. Print. Finn, Jennifer. Gods, Kings, Men: Trilingual Inscriptions and Symbolic Visualizations in the Achaemenid Empire. Ars Orientalis 41 (2011): 219-75. Print. Freeland, Travis, et al. Automated Feature Extraction for Prospection and Analysis of Monumental Earthworks from Aerial Lidar in the Kingdom of Tonga. Journal of Archaeological Science 69 (2016): 64-74. Print. Joye, Yannick, and Siegfried Dewitte. Up Speeds You Down. Awe-Evoking Monumental Buildings Trigger Behavioral and Perceived Freezing. Journal of Environmental Psychology 47.Supplement C (2016): 112-25. Print. Joye, Yannick, and Jan Verpooten. An Exploration of the Functions of Religious Monumental Architecture from a Darwinian Perspective. Review of General Psychology 17.1 (2013): 53-68. Print. McMahon, Augusta. Space, Sound, and Light: Toward a Sensory Experience of Ancient Monumental Architecture. American Journal of Archaeology 117.2 (2013): 163-79. Print. Stek, Tesse D. Monumental Architecture of Non-Urban Cult Places in Roman Italy. A Companion to Roman Architecture. Eds. Ulrich, Roger B. and Caroline K. Quenemoen. Hoboken, New Jersey: Wiley, 2014. 228-47. Print. Swenson, Edward. Moche Ceremonial Architecture as Thirdspace: The Politics of Place-Making in the Ancient Andes. Journal of Social Archaeology 12.1 (2012): 3-28. Print. Watkins, Trevor. New Light on Neolithic Revolution in South-West Asia. Antiquity 84.325 (2010): 621–34. Print.